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Tuesday, 01.23.2018, 02:56pm (GMT-7)
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GET TO KNOW YOUR NEIGHBOR ; SDEE 2015 Year End Social on Dec 8th in San Diego ; Must Attend 5-Day Event in San Diego Starting Tomorrow! -- CDW on Cells, Sensors, and Sysems, with Updates on Stem Cells ; SDEE October 2015 Event - Financings: Tales from the Tranches ; Allele Biotech Takes Major Step into Nano Antibody Leadership Position
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Imaging, Structure, and Optogenetics
Genetically encoded fluorescent thermosensors visualize subcellular thermoregulation in living cells
Monday, 10.14.2013, 11:08am
Genetically encoded sensors based on GFP enable the visualization of subcellular thermal changes noninvasively in intact cells.
Multicolor multicycle molecular profiling with quantum dots for single-cell analysis
Thursday, 09.19.2013, 09:00am
Here we present a detailed protocol for molecular profiling of individual cultured mammalian cells using multicolor multicycle immunofluorescence with quantum dot probes. It includes instructions for cell culture growth and processing (2 h + 48–72 h for cell growth), preparation and characterization of universal quantum
Quantitative imaging of disease signatures through radioactive decay signal conversion
Friday, 09.13.2013, 08:42am
Thorek et al. describe an activatable imaging agent based on a radioactive decay signal through the Cerenkov luminescence effect, which is light produced by β-particle–emitting radionuclides, such as clinically available PET tracers. The approach offers a means of simultaneously investigating multiple disease-relevant biological activities in vivo—the radioluminescent readout providing a quantitative measure of enzymatic activation with reduced background.
Label-Free Nanometer-Resolution Imaging of Biological Architectures through Surface Enhanced Raman Scattering
Wednesday, 09.11.2013, 10:23am
Label free imaging of the chemical environment of biological specimens would readily bridge the supramolecular and the cellular scales, if a chemical fingerprint technique such as Raman scattering can be coupled with super resolution imaging. We demonstrate the possibility of label-free super-resolution Raman imaging, by applying stochastic reconstruction to temporal fluctuations of the surface enhanced Raman scattering (SERS) signal which originate from biomolecular layers on large-area plasmonic surfaces with a high and uniform hot-spot density (>1011/cm2, 20 to 35 nm spacing). A resolution of 20 nm is demonstrated in reconstructed images of self-assembled peptide network and fibrilated lamellipodia of cardiomyocytes. Blink rate density is observed to be proportional to the excitation intensity and at high excitation densities (>10 kW/cm2) blinking is accompanied by molecular breakdown. However, at low powers, simultaneous Raman measurements show that SERS can provide sufficient blink rates required for image reconstruction without completely damaging the chemical structure.
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA
Monday, 08.12.2013, 12:42pm
Single DNA-binding proteins can be tracked on densely covered DNA at high spatial and temporal resolution and in the presence of high protein concentrations by using a technique that combines optical tweezers, confocal fluorescence microscopy and stimulated emission depletion (STED) nanoscopy.
Super-resolution Imaging of NPC in Living Cells
Tuesday, 07.30.2013, 01:40pm
Superresulotion imaging used to study the molecular organization of large protein complexes to well below 1 nanometer in whole cells.
  » Recombinant Probes for Visualizing Endogenous Synaptic Proteins in Living Neurons
  » Circularly polarized light detection by a chiral organic semiconductor transistor
  » Closed-loop optogenetic intervention in mice
  » In vivo imaging of glucose uptake and metabolism in tumors
  » Studying synapses in human brain with array tomography and electron microscopy
  » Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
  » Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms
  » Improved tools for the Brainbow toolbox
  » Real-time in vivo computed optical interferometric tomography
  » Real-time in vivo computed optical interferometric tomography

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High quality histologic services are crucial for determining study outcomes.
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EMPIAR: a public archive for raw electron microscopy image data
High-density three-dimensional localization microscopy across large volumes
Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases
A saposin-lipoprotein nanoparticle system for membrane proteins
Fabrication of nanopores with ultrashort single-walled carbon nanotubes inserted in a lipid bilayer
High-power sub-two-cycle mid-infrared pulses at 100 MHz repetition rate
SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data
Super-resolution links vinculin localization to function in focal adhesions
Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells
Computational high-resolution optical imaging of the living human retina