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GET TO KNOW YOUR NEIGHBOR ; SDEE 2015 Year End Social on Dec 8th in San Diego ; Must Attend 5-Day Event in San Diego Starting Tomorrow! -- CDW on Cells, Sensors, and Sysems, with Updates on Stem Cells ; SDEE October 2015 Event - Financings: Tales from the Tranches ; Allele Biotech Takes Major Step into Nano Antibody Leadership Position
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Imaging, Structure, and Optogenetics
Subwavelength anti-diffracting beams propagating over more than 1,000 Rayleigh lengths
Monday, 03.02.2015, 03:13pm
Researchers take advantage of the nonlinear response of a dipolar glass and observe non-paraxial propagation of a sub-micrometre-sized beam over 103 diffraction lengths.
Sub-100 nm resolution microscopy based on proximity projection grating scheme
Thursday, 02.26.2015, 11:02am
Here we present an approach to structured illumination microscopy using the proximity projection grating scheme (PPGS), which has the ability to further enhance the SIM resolution without invoking any nonlinearity response from the sample. With the PPGS-based SIM, sub-100 nm resolution has been obtained experimentally, and results corresponding to 2.4 times resolution improvement are presented.
Atomic-accuracy models from 4.5-Å cryo-electron microscopy data with density-guided iterative local refinement
Monday, 02.23.2015, 03:32pm
New detector technology has improved the resolution of cryo-electron microscopy (cryo-EM), but tools for structure determination from high-resolution maps have lagged behind. DiMaio et al. report structure determination from high-resolution cryo-EM maps using a homologous structure as a starting model. Also online, Wang et al. describe a de novo approach for structure determination that does not require a starting model.
Myosin II controls cellular branching morphogenesis and migration in three dimensions by minimizing cell-surface curvature
Friday, 02.13.2015, 12:26pm
Elliott et al. use high-resolution imaging, microfabrication and computational analyses to show that the interplay between cell-surface curvature and activity and cortical recruitment of myosin II, control cell migration in 3D matrices.
A general method to improve fluorophores for live-cell and single-molecule microscopy
Wednesday, 02.04.2015, 12:15pm
A simple and general chemical structure change to a panel of cell-permeable small-molecule fluorophores increases their brightness and photostability, which will enable improved single-molecule studies and super-resolution imaging.
Imaging liver biology in vivo using conventional confocal microscopy
Monday, 02.02.2015, 10:57pm
This protocol describes how to perform liver confocal intravital microscopy in mice, revealing the morphology and function of hepatocytes, endothelial cells and leukocytes in their native environment under physiological or pathological conditions.
  » Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms
  » Imaging liver biology in vivo using conventional confocal microscopy
  » Imaging liver biology in vivo using conventional confocal microscopy
  » Mechanistic insights into the recycling machine of the SNARE complex
  » Method of the Year 2014
  » Method of the Year 2014
  » Seeing in super-resolution
  » Nobel prize in physics: The birth of the blue LED
  » High-brightness table-top hard X-ray source driven by sub-100-femtosecond mid-infrared pulses
  » Time-reversed adapted-perturbation (TRAP) optical focusing onto dynamic objects inside scattering media

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High quality histologic services are crucial for determining study outcomes.
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EMPIAR: a public archive for raw electron microscopy image data
High-density three-dimensional localization microscopy across large volumes
Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases
A saposin-lipoprotein nanoparticle system for membrane proteins
Fabrication of nanopores with ultrashort single-walled carbon nanotubes inserted in a lipid bilayer
High-power sub-two-cycle mid-infrared pulses at 100 MHz repetition rate
SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data
Super-resolution links vinculin localization to function in focal adhesions
Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells
Computational high-resolution optical imaging of the living human retina