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GET TO KNOW YOUR NEIGHBOR ; SDEE 2015 Year End Social on Dec 8th in San Diego ; Must Attend 5-Day Event in San Diego Starting Tomorrow! -- CDW on Cells, Sensors, and Sysems, with Updates on Stem Cells ; SDEE October 2015 Event - Financings: Tales from the Tranches ; Allele Biotech Takes Major Step into Nano Antibody Leadership Position
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Imaging, Structure, and Optogenetics
 
 
EMPIAR: a public archive for raw electron microscopy image data
Wednesday, 03.23.2016, 05:06am

Recent advances in cryo-EM—for example, the development of direct-electron detectors—have led to a sharp increase in the number of 3D EM structures determined to high resolution.

 

High-density three-dimensional localization microscopy across large volumes
Friday, 03.18.2016, 09:44pm
Lattice light-sheet and PAINT microscopy are combined to achieve low-background detection of dense molecular labels, yielding super-resolution localization microscopy images of intricate 3D structures within dividing cells and embryos.
Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases
Wednesday, 03.16.2016, 12:12am
Here we report a hybrid crystal structure of Mycobacterium smegmatis mycocerosic acid synthase based on structures of its condensing and modifying regions. 
A saposin-lipoprotein nanoparticle system for membrane proteins
Tuesday, 03.15.2016, 12:05am
A saposin protein–lipid nanoparticle system stabilizes diverse, fragile membrane proteins in a lipid environment for structural and functional studies.
Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging
Saturday, 03.05.2016, 03:04am
Interferometric scattering microscopy (iSCAT) enables ultrasensitive label-free imaging and high-speed single-particle tracking. This protocol describes how to construct an iSCAT microscope with single-molecule TIRF capabilities.
Polarization modulation adds little additional information to super-resolution fluorescence microscopy
Thursday, 01.14.2016, 02:02am
a new set of states for super-resolution: the orientation of the emission dipoles of the fluorophores on the sample.
Sample preparation for high-resolution 3D confocal imaging of mouse skeletal tissue
Wednesday, 11.04.2015, 10:38am
This protocol describes how to prepare thick sections of mouse skeletal tissue for high-resolution 3D confocal imaging. Samples prepared in this way retain both fluorescence activity and antigenicity, allowing simultaneous imaging of multiple markers.
  » Monolayer excitonic laser
  » Fabrication of nanopores with ultrashort single-walled carbon nanotubes inserted in a lipid bilayer
  » Fabrication of nanopores with ultrashort single-walled carbon nanotubes inserted in a lipid bilayer
  » Optical control of excitation waves in cardiac tissue
  » Structural basis for gene regulation by a B12-dependent photoreceptor
  » High-power sub-two-cycle mid-infrared pulses at 100 MHz repetition rate
  » High-power sub-two-cycle mid-infrared pulses at 100 MHz repetition rate
  » SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data
  » SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data
  » Glycine receptor mechanism elucidated by electron cryo-microscopy


 
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High quality histologic services are crucial for determining study outcomes. http://www.allelebiotech.com/histology/
::| Hot News
EMPIAR: a public archive for raw electron microscopy image data
High-density three-dimensional localization microscopy across large volumes
Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases
A saposin-lipoprotein nanoparticle system for membrane proteins
Fabrication of nanopores with ultrashort single-walled carbon nanotubes inserted in a lipid bilayer
High-power sub-two-cycle mid-infrared pulses at 100 MHz repetition rate
SR-Tesseler: a method to segment and quantify localization-based super-resolution microscopy data
Super-resolution links vinculin localization to function in focal adhesions
Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells
Computational high-resolution optical imaging of the living human retina