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GET TO KNOW YOUR NEIGHBOR ; SDEE 2015 Year End Social on Dec 8th in San Diego ; Must Attend 5-Day Event in San Diego Starting Tomorrow! -- CDW on Cells, Sensors, and Sysems, with Updates on Stem Cells ; SDEE October 2015 Event - Financings: Tales from the Tranches ; Allele Biotech Takes Major Step into Nano Antibody Leadership Position
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FP Technologies and In Vivo Imaging
 
Fast, three-dimensional super-resolution imaging of live cells
Friday, 05.13.2011, 09:27am
Judicious choice of probes and imaging conditions allows two-dimensional super-resolution imaging of live cells at speeds up to 2 Hz with ~25-nm resolution and three-dimensional super-resolution imaging at ~1 Hz with ~30 nm x-y and ~50 nm z dimension resolution using stochastic optical reconstruction microscopy (STORM).
DAOSTORM: an algorithm for high- density super-resolution microscopy
Wednesday, 04.20.2011, 09:24am
Holden et al. showed that methods originally used to study crowded stellar fields can improve the performance of localization-based super-resolution microscopies, stochastic optical reconstruction microscopy (STORM), which use stochastic photoswitching to resolve closely spaced fluorophores and thus reconstruct super-resolved images, require that the specimen has a low density of active fluorophores.  Using the algorithm developed for astronomy, imaging speed and spatial resolution are significantly increased.
Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes
Friday, 03.04.2011, 11:15am
Confined photoactivation of photoactivatable mCherry using two-photon illumination with line-scanning temporal focusing in combination with three-dimensional localization algorithms allows three-dimensional super-resolution microscopy of cellular features at<50 nm lateral and<100 nm axial resolution and depths greater than 8 μm.
Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural expression patterns
Wednesday, 03.02.2011, 10:19am
A genetic multicolor cell-labeling technique for Droshophila melanogaster, Drosophila Brainbow, is described and applied to the study of neural circuits. This method implements a variant of the mouse Brainbow strategy in combination with specific neuronal targeting using the Gal-4–upstream activating sequence system to select for epitope-tagged proteins detectable with immunofluorescence. Also online, Hadjieconomou et al. develop a similar strategy, Flybow, to select for membrane-tethered fluorescent proteins.
Use of Fluorescent Protein in Studying Protein Half-Life
Monday, 02.14.2011, 09:00pm

One simple method was recently described in Science by Eden et al. that relies on bleaching fluorescent protein (FP) tagged to cellular protein of interest.

Photoactivable Fluorescent Proteins Used in Scanning EM
Monday, 01.03.2011, 01:49pm
EM researcher Jorgensen wanted to combine EM with recently developed super-resolution fluorescence microscope called stimulated emission depletion (STED), or a similar technique called photoactivated localization microscopy (PALM).
  » Warming up to a glow
  » The long road: peering into live cells (some with photoswitchable FPs)
  » Whole mouse organs imaged in 3D Using Self Fluorescence
  » Super-Imaging, Defy the Light Diffraction Limit
  » Which fluorescent protein is the best FRET donor?
  » BioTechnique: Recent papers advance GFP knowledge
  » How to use photoconvertible fluorescent proteins to trace dynamic subcellular structures


 
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::| Hot News
iSMS: single-molecule FRET microscopy software
[Research Article] Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light
Deep brain optical measurements of cell type–specific neural activity in behaving mice
Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate
Author File: Nathan Shaner, the Scintillon Institute
In vivo three-photon microscopy of subcortical structures within an intact mouse brain
Frequency-multiplexed in vivo multiphoton phosphorescence lifetime microscopy
A toolkit and benchmark study for FRET-restrained high-precision structural modeling
Manipulation of cellular light from green fluorescent protein by a femtosecond laser
Improving FRET dynamic range with bright green and red fluorescent proteins