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GET TO KNOW YOUR NEIGHBOR ; SDEE 2015 Year End Social on Dec 8th in San Diego ; Must Attend 5-Day Event in San Diego Starting Tomorrow! -- CDW on Cells, Sensors, and Sysems, with Updates on Stem Cells ; SDEE October 2015 Event - Financings: Tales from the Tranches ; Allele Biotech Takes Major Step into Nano Antibody Leadership Position
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FP Technologies and In Vivo Imaging
 
Improving FRET dynamic range with bright green and red fluorescent proteins
Monday, 09.10.2012, 09:36am
Development of the bright green and red fluorescent proteins, Clover and mRuby2, creates a fluorescence resonance energy transfer (FRET) pair with the highest Förster radius among existing ratiometric FRET pairs. Substitution of this pair for current FRET pairs in several existing sensors reliably and substantially improves sensor performance.
mWasabi is shown to be a brighter and better indicator
Tuesday, 09.04.2012, 09:08am
Compared with mRFP-EGFP-LC3, mTagRFP-mWasabi-LC3 has much brighter fluorescence, and is more accurate in monitoring autophagic flux.
Visualizing tumour propagation and metastasis in vivo
Wednesday, 07.25.2012, 06:56am
Using elegant in vivo imaging techniques, the Ignatius et al visualized embryonal rhabdomyosarcoma (ERMS) formation in the tail musculature of fluorescent transgenic zebrafish embryos microinjected with oncogenic transgenes, and identified distinct ERMS cell subpopulations that were fluorescently labelled based on the expression of different myogenic factors.

Their data suggest that non-tumor-propagating cells likely have important supportive roles in cancer progression and facilitate metastasis.
Rational design of true monomeric and bright photoactivatable fluorescent proteins
Friday, 06.29.2012, 02:45pm
Structure determination followed by targeted engineering of the popular photoactivatable fluorescent protein monomeric (m)Eos2 yields mEos3 versions that are more monomeric and less disruptive in protein fusions and also exhibit higher labeling density, brightness and other beneficial properties.
Single-cell systems biology by super-resolution imaging and combinatorial labeling
Monday, 06.04.2012, 09:19am
Super-resolution microscopy of fluorescently labeled oligonucleotides bound to individual mRNA transcripts is used for highly multiplexed imaging and quantification of transcripts in single cells. The method is used to profile transcripts from 32 stress-response genes in single yeast cells in response to extracellular stress.
Rational design of true monomeric and bright photoactivatable fluorescent proteins
Wednesday, 05.16.2012, 05:16pm
Structure determination followed by targeted engineering of the popular photoactivatable fluorescent protein monomeric (m)Eos2 yields mEos3 versions that are more monomeric and less disruptive in protein fusions and also exhibit higher labeling density, brightness and other beneficial properties.
  » Quantitative fluorescence labeling of aldehyde-tagged proteins for single-molecule imaging
  » Quantitative fluorescence labeling of aldehyde-tagged proteins for single-molecule imaging
  » Photoconvertible fluorescent protein used in identifying new long memory neurons
  » Localization imaging with standard fluorescent proteins on live cells—Bayesian modeling
  » Super-resolution 3D microscopy of live whole cells using structured illumination
  » Fluorescence imaging and reconstruction of transparent mouse brain
  » Two-photon polarization microscopy reveals protein structure and function
  » GFP Lasers
  » Identification of clustering artifacts in photoactivated localization microscopy
  » One particle to rule them all?


 
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::| Hot News
iSMS: single-molecule FRET microscopy software
[Research Article] Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light
Deep brain optical measurements of cell type–specific neural activity in behaving mice
Single-molecule evaluation of fluorescent protein photoactivation efficiency using an in vivo nanotemplate
Author File: Nathan Shaner, the Scintillon Institute
In vivo three-photon microscopy of subcortical structures within an intact mouse brain
Frequency-multiplexed in vivo multiphoton phosphorescence lifetime microscopy
A toolkit and benchmark study for FRET-restrained high-precision structural modeling
Manipulation of cellular light from green fluorescent protein by a femtosecond laser
Improving FRET dynamic range with bright green and red fluorescent proteins